8-iso-PGF2alpha EIA Kit

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Description Size Catalog# Price Quantity  
8-iso-PGF2alpha EIA Kit  1x96 well 900-010 $345.00  
8-iso-PGF2alpha EIA Kit  5x96 well 901-010 $1,395.00  
Background
The 8-epimer of Prostaglandin F2alpha (8-iso-PGF2alpha) is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependent mechanisms from arachidonic acid. 8-iso-PGF2alpha has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and atherosclerosis, and a mutagen in 3T3 and vascular smooth muscle cells. It has also been postulated to participate as a pathophysiological mediator, and is able to modify the fluidity and integrity of membranes. 8-iso-PGF2alpha has been shown to circulate in plasma and is excreted in urine. Methods for assessing total 8-iso-PGF2alpha typically require the alkaline hydrolysis of 8-iso-PGF2alpha esters from tissues, followed by lengthy procedures involving extractions, phase separations and thin layer chromatography.

Applications
EIA

Format
ELISA

Features

  • Results in just 3 hours

Range
6.1 - 100,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
8-iso-PGF2a (100%) / PGF1a (4.60%) / PGF2a (1.85%) / PGE1 (0.19%) / TXB2 (0.023%) / PGB1 ( 0.02%) / PGE3 (0.012%) / 6-keto-PGF1a (0.008%) / 13,14-dihydro-15-keto-PGF2a (0.008%) / 6,15-keto-13,14-dihydro-PGF1a (0.005%)... <0.001%: 8-iso-PGE1 / PGA2 / PGJ2 / 2-Arachidonoylglycerol / Anandamide

Sample Types
culture media, urine

Samples Per Kit
36 in duplicate per 96 well kit

Sensitivity
16.3 pg/mL

Significance and Uses
The regular 8-iso-PGF2alpha kit is a competitive immunoassay designed to measure free isoprostane in samples. The direct 8-iso-PGF2alpha measures total isoprostane levels, both free and esterified. This is accomplished by hydrolyzing the ester bond prior to analysis. The regular test uses culture media and urine as the sample types. Serum, heparin plasma, and EDTA plasma can be used in the direct test.

Species Reactivity
Species Independent

Time To Answer
< 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name Catalog # Manual
8-iso-PGF2alpha EIA Kit 900-010   Kit Insert    MSDS   
8-iso-PGF2alpha EIA Kit 901-010   Kit Insert    MSDS   

Ordering Info
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