Cortisol FPIA Kit

Applications
FPIA

Format
ELISA

Features

• Easy to use, homogeneous format
• Results in just 30 minutes

Range
781 - 800,000 pg/mL

Sample Size
100 µL/96 well, 40 µL/384 well

Product Image(s): click to enlarge

Cross Reactivities
11-Deoxycortisol 14%
Prednisolone 13%
Cortisone 9.1%
17alpha-Hydroxyprogesterone 2.5%
17-Hydroxypregnenolone 2.5%
Corticosterone 2.0%
Progesterone 1.1%
Androstenedione 0.5%
Prednisone 0.5%
17beta-Estradiol <0.1%
Testosterone <0.1%

Sample Types
cell supernatants

Samples Per Kit
100 tests at 100 µL

Sensitivity
685 pg/mL

Significance and Uses
Cortisol (hydrocortisone, compound F) is a steroid hormone synthesized from cholesterol. Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. Cortisol is found in the blood as either free cortisol, or more commonly, bound to corticosteroid-binding globulin (CBG). Serum levels are highest in the early morning and decrease throughout the day. Cortisol is involved primarily in metabolic and immunological actions. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, cortisol functions as an important anti-inflammatory and cortisol plays a role in hypersensitivity, immunosuppression and disease resistance. Factors such as age, various diseases, nutrition and other hormone levels affect cortisol secretion. It has also been shown that plasma cortisol levels elevate in response to stress. Abnormal cortisol levels are being tested for correlation with a variety of different conditions, including prostate cancer, depression and schizophrenia, while it is already known that an excess of cortisol in all bodily tissues is the cause of Cushings Syndrome.

Species Reactivity
Species Independent

Time To Answer
minimum of 30 minutes

Z Factor
0.823

For research use only, not for diagnostic or therapeutic use.