Cortisol EIA Kit

Product Comments
The Cortisol kit is a competitive immunoassay for the quantitative determination of Cortisol in biological fluids. Sample types include culture media, saliva, urine, serum, and plasma.

Applications
EIA

Format
ELISA

Features

• Highly cited in peer reviewed literature
• Excellent sensitivity
• Stable color coded reagents

Range
156 - 10,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
Cortisol 100%
Prednisolone 122.35%
Corticosterone 27.68%
11-deoxycortisol 4.0%
Progesterone 3.64%
Prednisone 0.85%
Testosterone 0.12%
Androstenedione <0.10%
Cortisone <0.10%
Estradiol <0.10%

Sample Types
culture media, saliva, urine, serum, plasma

Samples Per Kit
37 in duplicate per 96 well kit

Sensitivity
56.72 pg/mL

Significance and Uses
Cortisol (hydrocortisone, compound F) is a steroid hormone synthesized from cholesterol. Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. Cortisol is found in the blood as either free cortisol, or more commonly, bound to corticosteroid-binding globulin (CBG). Serum levels are highest in the early morning and decrease throughout the day. Cortisol is involved primarily in metabolic and immunological actions. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, cortisol functions as an important anti-inflammatory and cortisol plays a role in hypersensitivity, immunosuppression and disease resistance. Factors such as age, various diseases, nutrition and other hormone levels affect cortisol secretion. It has also been shown that plasma cortisol levels elevate in response to stress. Abnormal cortisol levels are being tested for correlation with a variety of different conditions, including prostate cancer, depression and schizophrenia, while it is already known that an excess of cortisol in all bodily tissues is the cause of Cushings Syndrome.

Species Reactivity
Species Independent

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.