Akt1,2 (phospho-Ser473) EIA Kit

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Description	Size	Catalog#	Price	Quantity
Akt1,2 (phospho-Ser473) EIA Kit	1x96 well	900-162	$525.00

Background

The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3beta, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has recently been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

Table of Abbreviations

Applications
EIA

Format
96-well plate

Product Image(s): click to enlarge

Features

Fast results in just 3 hours
Most sensitive assay commercially available
Fully validated in human, mouse, and rat cell lysates with both stimulation and inhibition experiments
Measures both Akt1 and Akt2 with nearly identical affinity
Reagents supplied to assay up to 41 samples in duplicate
Values normalized to percent phosphorylation of Akt1 at Serine 473

Range
17.5 - 560 pg/mL

Sample Size
100 無

Sensitivity
5.5 pg/mL

Sample Types
cell lysates

Samples Per Kit
41 in duplicate per 96 well kit

Species Reactivity
H, M, R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS

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Product Name	Catalog#
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Akt Polyclonal Antibody	KAP-PK004
Akt1 (PKB alpha) Monoclonal Antibody (5G12)	905-668
Akt1,2 Polyclonal Antibody	905-755-100
Akt1,2,3 (phospho-Ser473) Monoclonal Antibody (11E6)	905-658
Akt1,2,3 Monoclonal Antibody (11A11)	905-659
Akt1,2,3 Monoclonal Antibody (5C10)	905-661
Akt2 (PKB beta) Monoclonal Antibody (8B7)	905-660
Assay Buffer 32, 100 mL	80-1632
Hsp90 alpha ELISA Kit	EKS-895
PDK1 Polyclonal Antibody	KAP-PK112
PI3-kinase, p85 alpha Monoclonal Antibody (AB6)	KAM-PI200E
Stop Solution 2	80-0377
TMB Substrate	80-0350
Wash Buffer Concentrate	80-1287
mTOR Polyclonal Antibody	905-687-100

Citations
Launch Citation Database

Ordering Info
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Akt1,2 (phospho-Ser473) EIA Kit