GSK-3beta (total) EIA Kit

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Description	Size	Catalog#	Price	Quantity
GSK-3beta (total) EIA Kit	1x96 well	900-144	$525.00

Background

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Table of Abbreviations

Applications
EIA

Features

Results in just 3 hours
Sensitive to 74.4 pg/mL
Thoroughly validated in cell lysates

Range
78.1 - 5,000 pg/mL

Sample Size
100 無

Product Image(s): click to enlarge

Cross Reactivities
total GSK-3beta 100%
<0.01%: GSK-3alpha / ATP Citrate Lyase / MEK 1 / AKT / phosho JNK / ERK / phospho ERK

Sample Types
RIPA cell lysis buffer 2,2 million Jurkat cell/mL

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
74.4 pg/mL

Species Reactivity
H, R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout
Kit Insert
MSDS

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Citations
Launch Citation Database

Ordering Info
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GSK-3beta (total) EIA Kit