GSK-3beta (phospho) EIA Kit

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Description Size Catalog# Price Quantity  
GSK-3beta (phospho) EIA Kit 1x96 well  900-123 $525.00
Background
Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Table of Abbreviations

Applications
EIA

Features

  • Results in just 3 hours
  • Sensitive to 40.5 pg/mL

Range
62.5 - 2,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
phospho GSK-3beta 100%
<0.01%: GSK-3alpha / ATP Citrate Lyase / MEK / AKT / phosho JNK / ERK / phospho ERK

Sample Types
cell lysates

Samples Per Kit
41 in duplicate per 96 well kit

Sensitivity
24.1 pg/mL

Species Reactivity
H, R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout
Kit Insert
MSDS

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Citations
Launch Citation Database

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