13(S)-HODE EIA Kit

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Description	Size	Catalog#	Price	Quantity
13(S)-HODE EIA Kit	1x96 well	900-108	$360.00
13(S)-HODE EIA Kit	5x96 well	901-108	$1,440.00

Background

The major human dietary poly-unsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid via the lipoxygenase [13(S)-HODE] or P450 [13(R)-HODE] pathways or to 15-hydroxyeicosatetraenoic acid [15(S)-HETE] via the cyclooxygenase pathway. Changes in the intra-cellular ratio of 13(S)-HODE to 15(S)-HETE have been linked to a number of physiological conditions including cancer and cardiovascular disease. 13(S)-HODE helps to mediate cellular adhesion properties and excessive proliferation that is critical in the formation of tumors, metastases and atherogenic plaques. Continuously synthesized in non-stimulated vessel epithelia, 13(S)-HODE remains associated with the vitronectin receptor, a ubiquitous integrin adhesion molecule. Cell surfaces become adhesive when HODE concentrations decline, allowing the vitronectin receptor to relocate to the cell surface.

Applications
EIA

Format
ELISA

Features

Validated in numerous matrices
Highly cited in peer-reviewed literature

Range
3.9 - 1,000 ng/mL

Sample Size
100 無

Product Image(s): click to enlarge

Cross Reactivities
13(S)-HODE: 100%
13(R)-HODE: 2.00%
13-KODE: 0.48%
Linoleic Acid: 0.31%
gamma-Linolenic Acid: 0.28%
9(S)-HODE: 0.13%
12(R)-HETE: 0.07%
15(S)-HETE: 0.03%
<0.01%: 12(S)-HETE / Arachidonic Acid / DGLA / 5(S)-HETE / PGE₁

Sample Types
tissue culture media, serum, saliva, urine

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
1.6 ng/mL

Significance and Uses
The 13-hydroxyoctadecadienoic acid (13(S)-HODE) kit is a competitive immunoassay for the quantitative determination of free 13(S)-HODE in biological fluids. Sample types include culture media, heparin plasma, serum, saliva, and urine.

Species Reactivity
Species Independent

Time To Answer
4 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name	Catalog #	Manual
13(S)-HODE EIA Kit	900-108	Kit Insert MSDS
13(S)-HODE EIA Kit	901-108	Kit Insert MSDS

Related Products

Product Name	Catalog#
12(S)-HETE EIA Kit	900-050
15(S)-HETE EIA Kit	900-051
15(S)-HETE EIA Kit	901-051
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Creatinine Colorimetric Detection Kit	907-030
Cysteinyl Leukotriene EIA Kit	900-070
LTB4 EIA Kit	900-068

Citations
Launch Citation Database

Ordering Info
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Order by phone: 734-668-6113 or 877-423-4548
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Order by fax: 734-669-0134

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13(S)-HODE EIA Kit