cyclic GMP Complete EIA Kit

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Description Size Catalog# Price Quantity  
cyclic GMP Complete EIA Kit  1x96 well 900-164 $350.00  
cyclic GMP Complete EIA Kit  5x96 well 901-164 $1,450.00  
Background
Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

  • Accurately quantitate cGMP in biological fluids, cells, and tissue with one assay.
  • Acetylation option increases sensitivity 10-fold.
  • Ready-to-use liquid color coded reagents reduce technician error while saving time and money.

Range
0.8 - 500 pmol/mL (non-acetylated in Assay Buffer); 0.08 - 50 pmol/mL (acetylated in Assay Buffer)
0.8 - 500 pmol/mL (non-acetylated in Assay Buffer); 0.08 - 50 pmol/mL (acetylated in Assay Buffer)
0.8 - 500 pmol/mL (non-acetylated in Assay Buffer); 0.08 - 50 pmol/mL (acetylated in Assay Buffer)
0.8 - 500 pmol/mL (non-acetylated in 0.1M HCl); 0.08 - 50 pmol/mL (acetylated in 0.1M HCl)

Cross Reactivities
cGMP 100%; GMP, GTP, cAMP, AMP, ATP, cUMP, CTP <0.001%

Sample Types
CL, CS, P, S, SA, T, U

Samples Per Kit
39 samples in duplicate per 96 wells

Sensitivity
Assay Buffer : 0.42 pmol/mL (non-acetylated) 0.043 pmol/mL (acetylated) HCl: 0.604 pmol/mL (non-acetylated) 0.059 pmol/mL (acetylated)

Species Reactivity
Species Independent

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout

Product Manual

Product Name Catalog # Manual
cyclic GMP Complete EIA Kit 900-164   Kit Insert    MSDS   
cyclic GMP Complete EIA Kit 901-164   Kit Insert    MSDS   

Ordering Info
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