Leptin (human) EIA Kit

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Description Size Catalog# Price Quantity  
Leptin (human) EIA Kit 1x96 well  900-028 $475.00
Background
The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic beta cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-alpha (inhibition). Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood.

Table of Abbreviations

Applications
EIA

Features

  • Results in just 2 hours
  • Convenient 4°C storage

Range
195 - 12,500 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
human Leptin 100%
mouse Leptin 0.3%
0.10%: rat Leptin / human ET-1 / human VEGF / human GRO-alpha / human SCF / human TPO

Sample Types
culture media, EDTA plasma, serum

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
25.5 pg/mL

Species Reactivity
H

Time To Answer
2 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout
Kit Insert
MSDS

Related Products

Product Name Catalog#
Leptin (mouse) EIA Kit
 		900-019
Leptin (rat) EIA Kit
 		900-015

Citations
Launch Citation Database

Ordering Info
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