Grp94 ImmunoSet Kit

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Description Size Catalog# Price Quantity  
Grp94 ImmunoSet Kit   5x96 well 960-077 $450.00  
Background
What is an ImmunoSet?

Glucose-regulated protein 94 (Grp94, gp96), an abundant resident endoplasmic reticulum (ER) lumenal stress protein, belongs to the Hsp90 family of molecular chaperones along with cytosolic Hsp90. Grp94 possess the C-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal responsible for its retention in pre-Golgi compartments. Stress conditions such as glucose starvation and heat shock which promote protein misfolding or unfolding increase Grp94 expression. In addition to a homeostatic role in protein folding and assembly, Grp94 functions in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway in antigen presenting cells.

Table of Abbreviations

Applications
EIA

Species Reactivity
H, M, R, C

Features

  • Cost effective- coat your own plates and stretch your research budget
  • Quantitative - obtain fully quantitative statistically significant results
  • Sensitive - detect as little as 1.29 ng/mL Grp94 compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents in multiple complex sample matrices

Range
6.25 - 200 ng/mL

Sample Size
100 µL

Cross Reactivities
This assay detects Grp94 in cell lysates and microsomes. Cross reactivity with human Calreticulin, PDI, Hsp90 alpha, Hsp90 beta, Hsp70, hamster Grp78 and bovine Hsc70 is less than 0.035%.

Sensitivity
1.29 ng/mL

Sample Types
CL, T

Samples Per Kit
~200 in duplicate

Time To Answer
Plate Coating - Overnight + 1 hrs; Assay - 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert
MSDS

Product Manual

Product Name Catalog # Manual
Grp94 ImmunoSet Kit 960-077  

Ordering Info
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