PDI ImmunoSet Kit

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Description Size Catalog# Price Quantity  
PDI ImmunoSet Kit   5x96 well 960-072 $450.00  
Background
What is an ImmunoSet?

The mammalian protein disulphide-isomerase (PDI) family encompasses several highly divergent proteins involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulphide bonds. PDI, an abundant protein of the ER (>400µM), contains a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94. The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin.

Table of Abbreviations

Applications
EIA

Species Reactivity
H, M, R

Features

  • Cost effective - coat your own plates and stretch your research budget
  • Quantitative - obtain fully quantitative statistically significant results
  • Sensitive - detect as little as 3.93 ng/mL PDI compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents in complex sample matrices

Range
7.8 - 250 ng/mL

Sample Size
100 µL

Cross Reactivities
PDI 100%... Grp94 0.121%, Hsc70 0.018%... Calreticulin, Grp78, Hsp90alpha, Hsp90beta, and Hsp70 0.016%

Sensitivity
3.93 ng/mL

Sample Types
CL, P, T

Samples Per Kit
~200 in duplicate

Time To Answer
Plate Coating - Overnight + 1 hrs; Assay - 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert
MSDS

Product Manual

Product Name Catalog # Manual
PDI ImmunoSet Kit 960-072  

Ordering Info
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