Hsp25 ImmunoSet Kit

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Description Size Catalog# Price Quantity  
Hsp25 ImmunoSet Kit   5x96 well 960-075 $450.00  
Background
What is an ImmunoSet?

Mouse Hsp25, human Hsp27, and alphaB-crystallin are part of a diverse family of small heat shock proteins (sHsps) that range in size from 14-45 kDa. sHsps are constitutively expressed, and upregulated in response to stress such as heat shock. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Data indicates that Hsp25 is a dynamic tetramer of tetramers with a unique ability to refold and reassemble into its active quaternary structure after denaturation. Hsp25 helps facilitate the glutathione-redox cycle by enhancing glutathione utilization and maintaining the cellular glutathione pool in favor of the reduced states.

Table of Abbreviations

Applications
EIA

Species Reactivity
M, R

Features

  • Cost effective - coat your own plates and stretch your research budget
  • Quantitative - obtain fully quantitative statistically significant results
  • Sensitive - detect as little as 380 pg/mL HO-1 compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents in multiple complex sample matrices

Range
0.78 - 25 ng/mL

Cross Reactivities
Specific for Hsp25 - does not cross-react with Hsp27, Cpn10, HO-1, Hsp40, or alphaB-crystallin

Sensitivity
0.38 ng/mL

Sample Types
CL

Samples Per Kit
~200 in duplicate

Time To Answer
Plate Coating - Overnight + 1 hour; Assay - 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert
MSDS

Product Manual

Product Name Catalog # Manual
Hsp25 ImmunoSet Kit 960-075  

Ordering Info
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