Hsp27 High Sensitivity ImmunoSet Kit

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Description Size Catalog# Price Quantity  
Hsp27 High Sensitivity ImmunoSet Kit   5x96 well 960-076 $450.00  
Background
What is an ImmunoSet?

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Table of Abbreviations

Applications
EIA

Species Reactivity
H

Features

  • Cost effective - coat your own plates and stretch your research budget
  • Quantitative - obtain fully quantitative statistically significant results
  • Ultra-Sensitive - detect as little as 97 pg/mL Hsp27 compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents in multiple complex sample matrices

Range
0.10 - 3.2 ng/mL

Sample Size
100 µL

Cross Reactivities
Specific for Hsp27. Does not cross-react with Hs25, Cpn10, HO-1, Hsp40, or alphaB-crystallin.

Sensitivity
97 pg/mL

Sample Types
CL, P, S

Samples Per Kit
~200 in duplicate

Time To Answer
Plate Coating - Overnight + 1 hrs; Assay - 2 hours 45 minutes

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert
MSDS

Product Manual

Product Name Catalog # Manual
Hsp27 High Sensitivity ImmunoSet Kit 960-076  

Related Products

Product Name Catalog#
Hsp27 (phospho-Ser15) EIA Kit
900-170
Hsp27 (phospho-Ser78) EIA Kit
900-165
Hsp27 ELISA Kit
EKS-500
ImmunoSet Buffer Pack
950-003
ImmunoSet Buffer Pack
950-003
ImmunoSet Plate Pack
80-1930

Citations
Launch Citation Database

Ordering Info
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