HO-1 (rat) ImmunoSet Kit

Brand: Assay Designs Print Page
Description Size Catalog# Price Quantity  
HO-1 (rat) ImmunoSet Kit   5x96 well 960-810 $450.00  
Background
What is an ImmunoSet?

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

Table of Abbreviations

Applications
EIA

Species Reactivity
R

Features

  • Cost effective - coat your own plates and stretch your research budget
  • Quantitative - obtain fully quantitative statistically significant results
  • Sensitive - detect as little as 390 pg/mL HO-1 compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents using rat cell lysates, plasma, serum, and tissue

Range
0.195 - 12.5 ng/mL

Sample Size
100 µL

Cross Reactivities
<0.02%: rat HO-2 / mouse HO-1 / human HO-1

Sensitivity
0.039 ng/mL

Sample Types
CL, P, S, T

Samples Per Kit
200 in duplicate

Time To Answer
Plate Coating - Overnight + 1 hour; Assay - 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
CofA_960-810_08130802
Kit Insert
MSDS

Product Manual

Product Name Catalog # Manual
HO-1 (rat) ImmunoSet Kit 960-810  

Related Products

Product Name Catalog#
HO-1 (Hsp32) Monoclonal Antibody (HO-1-1)
OSA-110
HO-1 (Hsp32) Polyclonal Antibody
SPA-895
HO-1 (Hsp32) Polyclonal Antibody
SPA-896
HO-1 (Hsp32) Recombinant Rat Protein
SPP-730
HO-1 (Rat) ELISA Kit
EKS-810A
HO-1 (human) ImmunoSet Kit
960-800
HO-1 (mouse) ImmunoSet Kit
960-071
ImmunoSet Buffer Pack
950-003
ImmunoSet Plate Pack
80-1930

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
 Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134