cyclic GMP Complete EIA Kit

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Description Size Catalog# Price Quantity  
cyclic GMP Complete EIA Kit   1x96 well 900-164 $350.00  
cyclic GMP Complete EIA Kit   5x96 well 901-164 $1,450.00  
Background
The Assay Designs cGMP Complete ELISA is a flexible assay to measure either intra- or extracellular cGMP in a wide variety of sample matrices. An acid solution is provided to prepare cell and tissue samples to stop endogenous phosphodiesterase activity. Aceltylation reagents are also provided to offer an increase in assay sensitivity.

Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.

Normalize urine samples to creatinine with our Creatinine Colorimetric Detection Kit.

Table of Abbreviations

Applications
EIA

Species Reactivity
Species Independent

Features

  • Versatile - one convenient ELISA to assay both intracellular and extracellular cGMP
  • Simple - easy-to-use liquid color-coded reagents reduce errors
  • Sensitive - measure as little as 43 fmol/mL cGMP

Range
0.8 - 500 pmol/mL (non-acetylated in Assay Buffer); 0.08 - 50 pmol/mL (acetylated in Assay Buffer)
0.8 - 500 pmol/mL (non-acetylated in Assay Buffer); 0.08 - 50 pmol/mL (acetylated in Assay Buffer)
0.8 - 500 pmol/mL (non-acetylated in Assay Buffer); 0.08 - 50 pmol/mL (acetylated in Assay Buffer)
0.8 - 500 pmol/mL (non-acetylated in 0.1M HCl); 0.08 - 50 pmol/mL (acetylated in 0.1M HCl)

Cross Reactivities
cGMP 100%; GMP, GTP, cAMP, AMP, ATP, cUMP, CTP <0.001%

Sample Types
CL, CS, P, S, SA, T, U

Samples Per Kit
39 samples in duplicate per 96 wells

Sensitivity
Assay Buffer : 0.42 pmol/mL (non-acetylated) 0.043 pmol/mL (acetylated) HCl: 0.604 pmol/mL (non-acetylated) 0.059 pmol/mL (acetylated)

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout

Product Manual

Product Name Catalog # Manual
cyclic GMP Complete EIA Kit 900-164   Kit Insert     MSDS    
cyclic GMP Complete EIA Kit 901-164   Kit Insert     MSDS    

Ordering Info
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Order by fax: 734-669-0134