15(S)-HETE EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
15(S)-HETE EIA Kit	1x96 well	900-051	$360.00
15(S)-HETE EIA Kit	5x96 well	901-051	$1,440.00

Background

HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

Normalize urine samples to creatinine with our Creatinine Colorimetric Detection Kit.

Table of Abbreviations

Applications
EIA

Species Reactivity
Species Independent

Features

Most sensitive 15(S)-HETE kit on the market
Few preparation steps
Much easier than GC/MS measurement

Range
78.1 - 20,000 pg/mL

Sample Size
100 無

Product Image(s): click to enlarge

Cross Reactivities
15(S)-HETE 100%
8,15-diHETE 1.00%
5,15-diHETE 1.00%
13(S)-HODE 0.60%
5-HETE 0.10%
PGD2 0.10%
PGF2alpha 0.10%
PGB2 0.10%
12(S)-HETE <0.05%
Arachidonic Acid <0.05%
PGE2 <0.05%
12(R)-HETE <0.05%
Linoleic Acid <0.05%

Sample Types
culture media, urine, EDTA plasma, serum

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
69.21 pg/mL

Time To Answer
Overnight + 1 hour

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name	Catalog #	Manual
15(S)-HETE EIA Kit	900-051	Kit Insert MSDS
15(S)-HETE EIA Kit	901-051	Kit Insert MSDS

Related Products

Product Name	Catalog#
12(S)-HETE EIA Kit	900-050
12(S)-HETE EIA Kit	901-050
13(S)-HODE EIA Kit	900-108
15(S)-HETE EIA Kit	900-051
15(S)-HETE EIA Kit	901-051
Creatinine Colorimetric Detection Kit	907-030
Cysteinyl Leukotriene EIA Kit	900-070
LTB4 EIA Kit	900-068
PGE2 EIA Kit	900-001
PGF2alpha EIA Kit	900-069
TXB2 EIA Kit	900-002

Citations
Launch Citation Database

Ordering Info
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Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134

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15(S)-HETE EIA Kit