uNOS Polyclonal Antibody

Brand: Assay Designs Print Page
Description Size Catalog# Price Quantity  
uNOS Polyclonal Antibody 1 mL  905-611 $305.00
Background
Nitric oxide (NO) is synthesized from L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthases (NOS I-III). Analysis of cDNA clones has identified three distinct NOS genes in mammals: neuronal (nNOS/NOS-I), endothelial (eNOS/NOS-III), and inducible (iNOS/NOS-II). eNOS and nNOS are constitutively expressed, agonist-triggered, Ca2+/calmodulin-dependent, and are inhibited by L-arginine analogues (L-NNA, L-NMMA). They are expressed in endothelium, adrenal glands, brain, and platelets. iNOS is inducible, Ca2+/calmodulin-independent, and also inhibited by NMMA and L-NNA. It is expressed in macrophages, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.

Table of Abbreviations

Applications
IHC, WB

Format
PBS, pH 7.4, with BSA and sodium azide

Product Image(s): click to enlarge

Source Type
Rabbit pAb

Immunogen
Synthetic peptide derived from sequence near the carboxy-terminus of mouse iNOS and nNOS

Positive Control
human placenta

Purification Method
Peptide Affinity

Species Reactivity
H, M, R, B, P

Significance & Uses
NOS ( Nitric Oxide Synthase) oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134