Methyltransferase Activity Kit

Brand: Assay Designs Print Page
Description Size Catalog# Price Quantity  
Methyltransferase Activity Kit 1x96 well   907-025 $385.00
Background
The Assay Designs Methyltransferase Activity Kit is intended for screening candidate compounds that may alter normal methyltransferase activity and is suitable for use with all S-adenosylmethionine dependent methyltransferases. Methyltransferase activity is measured without the need for radiolabeled samples with both endpoint and kinetic assay options available for fluormetric detection (380ex/520em). Common solvents have been tested for interference in the assay.

The methylation of proteins, xenobiotic drugs, nucleic acids, and oligosaccharides is known to regulate important cellular events including meiosis, biosynthesis, development, signal transduction, chromatin remodeling, and gene silencing. The most frequent methyl donor for this type of enzymatic modification is S-adenosyl-L-methionine (AdoMet/SAM). Based on the predicted binding motif for AdoMet, it is estimated that 1% of the mammalian genome encodes a variety of substrate-specific methyltransferases.

Table of Abbreviations

Applications
Activity

Species Reactivity
Species Independent

Product Image(s): click to enlarge

Features

  • Universal - suitable for any SAM-dependent MT
  • Robust - Z factor = 0.83
  • Sensitive - fluorescent detection allows for both DNA and protein assays
  • Simple - homogeneous mix-and-read plate-based assay

Sample Types
Purified in vitro samples that do not contain reductants

Time To Answer
30 min (Endpoint assay option); Kinetic assay option is dependenent upon samples

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS
Methyltransferase Poster (6.5 MB)

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Citations
Launch Citation Database

Ordering Info
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Order by fax: 734-669-0134