GSK-3 beta Polyclonal Antibody

Brand: Assay Designs Print Page
Description Size Catalog# Price Quantity  
GSK-3 beta Polyclonal Antibody  100 µg 905-679-100 $295.00  
Background
Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Table of Abbreviations

Abbreviated Name
GSK-3 beta pAb

Applications
WB, IHC

Species Reactivity
H, M, R, B

Positive Controls
HeLa Lysates, Catalog Number LYC-HL100F; Human Jurkat cells; Mouse 3T3 cells; Rat PC-12 cells; Bovine MDBK cells

Product Image(s): click to enlarge

Immunogen
Synthetic peptide derived from sequence near the carboxy - terminus of human GSK3â

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Product Manual

Product Name Catalog # Manual
GSK-3 beta Polyclonal Antibody 905-679-100  

Related Products

Product Name Catalog#
GSK-3 alpha/beta (phospho-Tyr216/Tyr279) Polyclonal Antibody
 		KAP-ST012
GSK-3 alpha/beta Monoclonal Antibody (1H8)
 		KAM-ST002
GSK-3 beta (phospho-Ser9) Monoclonal Antibody (3A8)
 		905-761-100
GSK-3 beta (phospho-Ser9) Polyclonal Antibody
 		KAP-ST011
GSK-3 beta Monoclonal Antibody (11B9)
 		905-760-100
GSK-3 beta Polyclonal Antibody
 		KAP-ST002
GSK-3 beta Recombinant Human Protein
 		PPK-425
GSK-3beta (phospho) EIA Kit
 		900-123
GSK-3beta (total) EIA Kit
 		900-144

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
 Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134