Akt2 (PKB beta) Monoclonal Antibody (8B7)

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Description Size Catalog# Price Quantity  
Akt2 (PKB beta) Monoclonal Antibody (8B7) 100 µg  905-660 $385.00
Background
The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3beta, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has recently been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

Table of Abbreviations

Abbreviated Name
Akt2 mAb (8B7)

Conjugate

Isotype
IgG2a

Product Image(s): click to enlarge
western blot

Applications
EIA, IHC, WB

Purification
Thiophilic adsorption and size exclusion chromatography

Immunogen
Synthetic peptide derived from the sequence of Akt2 (PKB beta), conjugated to KLH

Host
Mouse

Clone Number
8B7

Species Reactivity
H

Positive Control
Untreated HepG2 cells

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Related Products

Product Name Catalog#
Akt (PKB) Kinase Activity Kit
 		EKS-400A
Akt1,2 (phospho-Ser473) EIA Kit
 		900-162
Akt1,2 Polyclonal Antibody
 		905-755-100
Akt2 (PKB beta) Polyclonal Antibody
 		KAP-PK008E

Citations
Launch Citation Database

Ordering Info
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