Hsp27 (phospho-Ser82) Monoclonal Antibody (5B9)

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Description Size Catalog# Price Quantity  
Hsp27 (phospho-Ser82) Monoclonal Antibody (5B9) 100 µg  905-642 $395.00
Background
Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Table of Abbreviations

Abbreviated Name
Hsp27 phospho-Ser82 mAb (5B9)

Conjugate

Isotype
IgG2a

Product Image(s): click to enlarge

Applications
IHC, WB

Purification
Thiophilic adsorption and size exclusion chromatography

Immunogen
Synthetic phospho-peptide derived from the sequence of human Hsp27, conjugated to KLH

Host
Mouse

Clone Number
5B9

Species Reactivity
H, M, R, C

Positive Control
EGF-treated HepG2 cells

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Related Products

Product Name Catalog#
Hsp27 (phospho) Recombinant Human Protein
 		SPP-716
Hsp27 (phospho-Ser15) Polyclonal Antibody
 		SPA-525
Hsp27 (phospho-Ser78) Polyclonal Antibody
 		SPA-523
Hsp27 (phospho-Ser82) Polyclonal Antibody
 		SPA-524

Citations
Launch Citation Database

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