GSK-3 alpha/beta (phospho-Tyr216/Tyr279) Polyclonal Antibody

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
GSK-3 alpha/beta (phospho-Tyr216/Tyr279) Polyclonal Antibody  100 µL KAP-ST012E $325.00  
Background
Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Table of Abbreviations

Abbreviated Name
GSK-3 alpha/beta phospho-Tyr216/Tyr279 pAb

Conjugate

Applications
EIA, WB

Isotype
not applicable

Purification
Peptide Immunoaffinity

Immunogen
Human GSK-3â synthetic peptide containing phosphorylated tyrosine 216/279.

Host
Rabbit

Clone Number
not applicable

Product Image(s): click to enlarge

Species Reactivity
H, M (rat and zebra fish GSK-3â, and rat and Xenopus GSK-3â were not tested but predicted to react based on 100% sequence homology )

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Product Manual

Product Name Catalog # Manual
GSK-3 alpha/beta (phospho-Tyr216/Tyr279) Polyclonal Antibody KAP-ST012E  

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Citations
Launch Citation Database

Ordering Info
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