GSK-3 alpha/beta Monoclonal Antibody (1H8)

Brand: Stressgen

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Description	Size	Catalog#	Price	Quantity
GSK-3alpha/beta Monoclonal Antibody (1H8)	50 痢	KAM-ST002D	$150.00
GSK-3alpha/beta Monoclonal Antibody (1H8)	200 痢	KAM-ST002F	$350.00

Background

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Table of Abbreviations

Abbreviated Name
GSK-3 alpha/beta mAb (1H8)

Conjugate

Applications
WB

Isotype
IgG2a

Purification
Protein G Affinity

Immunogen
Recombinant Xenopus GSK-3beta protein

Host
Mouse

Clone Number
1H8

PositiveControls
HeLa Cell Lysate (Heat Shocked), LYC-HL101 Mouse Brain Tissue Extract, LYT-MB100 Rat Brain Tissue Extract, LYT-RB100

Species Reactivity
H, M, R, B, C, HA, MO, P, RB, S

For research use only, not for diagnostic or therapeutic use.

Documentation
MSDS
Spec Sheet

Product Manual

Product Name	Catalog #	Manual
GSK-3alpha/beta Monoclonal Antibody (1H8)	KAM-ST002D
GSK-3alpha/beta Monoclonal Antibody (1H8)	KAM-ST002F

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GSK-3 alpha/beta (phospho-Tyr216/Tyr279) Polyclonal Antibody	KAP-ST012
GSK-3 beta Polyclonal Antibody	905-679-100
GSK-3beta (phospho) EIA Kit	900-123
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HeLa Cell Lysate, Heat Shocked	LYC-HL101F

Citations
Launch Citation Database

Ordering Info
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GSK-3 alpha/beta Monoclonal Antibody (1H8)