DNA Damage ELISA Kit

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
DNA Damage ELISA Kit 1x96 well  EKS-350 $720.00
Background
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage. Assay Designs? COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic ?comet? shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA. The Stressgen® DNA Damage Kit is a competitive ELISA for the detection and quantification of 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidized nucleoside of DNA that is excised from damaged DNA and present in urine and serum. 8-OHdG is a frequently used biomarker of oxidative DNA damage and oxidative stress, and may serve as a risk factor for cancer, atherosclerosis and diabetes.

Table of Abbreviations

Applications
EIA

Range
0.94 - 60 ng/mL

Sample Size
50µL/well

Sample Types
urine, serum, and saliva

Samples Per Kit
39 samples in duplicate per 96 well kit

Sensitivity
0.59 ng/mL

Species Reactivity
Species Independent

Time To Answer
< 2.5 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
DNA Damage Calculation Sheet (Excel file)
Kit Insert
Technical Bulletin

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Citations
Launch Citation Database

Ordering Info
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