Hsp70 ELISA Kit

Brand: Stressgen

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Description	Size	Catalog#	Price	Quantity
Hsp70 ELISA Kit	1x96 well	EKS-700B	$540.00

Background

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

The first commercially available Hsp70 assay on the market
Hundreds of publications in peer-reviewed journals
Suitable for cell and tissue extracts

Range
780 - 50,000 pg/mL

Cross Reactivities
Specific for both natural source and recombinant inducible Hsp70. The assay does not cross react with 5,000ng/mL of bovine constitutive Hsc70, recombinant hamster Grp78, E. coli DnaK, or recombinant M. tuberculosis Hsp71.

Sample Size
100無/well

Product Image(s): click to enlarge

Sample Types
cell lysates, and tissue extracts

Samples Per Kit
40 samples in duplicate per 96 well kit

Sensitivity
200 pg/mL

Species Reactivity
H, M, R

Time To Answer
4.5 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert

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Stop Solution 2	80-0377
TMB Substrate	80-0350
Wash Buffer Concentrate	80-1287

Citations
Launch Citation Database

Ordering Info
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Hsp70 ELISA Kit