p38 (phospho) EIA Kit

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Description Size Catalog# Price Quantity  
p38 (phospho) EIA Kit 1x96 well  900-101 $525.00
Background
Six proteins of the p38 subfamily of MAP kinases have been identified to date, including: p38alpha (SAPK2, CSBP) and its splice variant Mxi; p38beta and beta2; p38gamma (ERK6, SAPK3); and p38delta (SAPK4). p38 MAP kinases contain a conserved TGY motif in their activation loop and are activated in response to cytokines, hormones, GPCRs, and osmotic and heat shock to mediate primarily pro-apoptotic and pro-inflammatory responses. MKK3, MKK4, and MKK6 are known to activate p38 family members with varying specificity. All four p38 isoforms are able to phosphorylate the activating transcription factor 2 (ATF2), and differentially activate numerous substrates including Mnk1/2, Msk, phospholipase A2, and the MAPKAP kinase-2 and -3. Activated MAPKAP kinase-2 subsequently phosphorylates the heat shock protein Hsp27, an F-actin binding protein involved in cytoskeletal maintenance.

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

  • Results in just 3 hours
  • Much easier than Western blotting

Range
156 - 5,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
phospho p38 100%
< 0.01%: non-phosphorylated p38 / phospho JNK / non-phosphorylated JNK / AKT / non-phosphorylated ERK / phospho ERK

Sample Types
RIPA cell lysis buffer

Samples Per Kit
40 in duplicate per 96 well kit

Sensitivity
52.1 pg/mL

Species Reactivity
H, M

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout
Kit Insert
MSDS

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Citations
Launch Citation Database

Ordering Info
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