cyclic AMP (direct) EIA Kit

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Description Size Catalog# Price Quantity  
cyclic AMP (direct) EIA Kit   1x96 well 900-066 $335.00  
cyclic AMP (direct) EIA Kit   5x96 well 901-066 $1,395.00  
Background
The Assay Designs cAMP (direct) ELISA is an ultrasensitive assay to measure as little as 0.037 pmol/mL cyclic AMP. The kit comes complete with an easy-to-use acid solution to lyse cells and prepare tissue samples to stop endogenous phosphodiesterase activity. Acetylation reagents are also provided as an option to increase assay sensitivity 10-fold.

Cyclic AMP (cAMP) is one of the most important second messengers involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

Assay Designs also provides cyclic AMP EIA kits in a convenient 384 well format upon request. Please contact info@assaydesigns.com for additional information.

Table of Abbreviations

Applications
EIA

Species Reactivity
Species Independent

Features

  • Trusted - highly cited in peer reviewed literature
  • Flexible - can be run acetylated to increase sensitivity ~10-fold
  • Fast- results in just 3 hours
  • Easy-to-use - liquid color-coded reagents reduce errors

Range
(non-acetylated) 0.78 - 200 pmol/mL; (acetylated) 0.078 - 20 pmol/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
cyclic AMP 100%
AMP 0.33%
ATP 0.12%
<0.001%: cGMP, GMP, GTP, cUMP, CTP

Sample Types
cells, tissue, culture supernates

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
(non-acetylated) 0.39 pmol/mL; (acetylated) 0.037 pmol/mL

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name Catalog # Manual
cyclic AMP (direct) EIA Kit 900-066   Kit Insert     MSDS    
cyclic AMP (direct) EIA Kit 901-066   Kit Insert     MSDS    

Ordering Info
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Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134