Flow cytometry is a common technique used to count, characterize, and often sort cells based on a variety of criteria including size, intracellular complexity, and fluorescence following pre-treatment of the cells with fluorescent dyes or fluorochrome-conjugated antibodies against specific antigens. Flow cytometry can be used to differentiate between cell types, particularly cells of lymphoid origin, using antibodies against lineage-specific cell surface markers. Permeabilization of cell membranes prior to antibody staining allows investigators to assess the expression of intracellular proteins as well.
Assay Designs™ offers a variety of flow cytometry validated antibodies against both extracellular and intracellular antigens, including Stressgen® antibodies for markers of cellular stress.
Many antibodies and isotype controls are available as fluorochrome conjugates to FITC, DyLight™ 488, or phycoerythrin (PE).
If you are unable to find the conjugate you need, Assay Designs also offers custom conjugation of our antibodies to a variety of common fluorophores and enzymes.
Flow cytometry is a common technique used to count, characterize, and often sort cells based on a variety of criteria including size, intracellular complexity, and fluorescence following pre-treatment of the cells with fluorescent dyes or fluorochrome-conjugated antibodies against specific antigens. Flow cytometry can be used to differentiate between cell types, particularly cells of lymphoid origin, using antibodies against lineage-specific cell surface markers. Permeabilization of cell membranes prior to antibody staining allows investigators to assess the expression of intracellular proteins as well.
